Molecular Typing of Staphylococcus Aureus Strains from Ovine Mastitis by Pulsed-Field Gel Electrophoresis and Polymerase Chain Reaction Based on Coagulase and Protein A Gene Polymorphisms
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چکیده
منابع مشابه
Molecular typing of nosocomial Staphylococcus aureus strains associated to biofilm based on the coagulase and protein A gene polymorphisms
Objective(s): Staphylococcus aureus is an important bacterial pathogen responsible for a variety numbers of nosocomial and community acquired infections. Biofilm formation is regarded as an important factor in the establishment of S. aureus infection. The contribution of the genetic background of S. aureus to biofilm formation is poorly understood. The aim of the present work was to genotype S....
متن کاملmolecular typing of nosocomial staphylococcus aureus strains associated to biofilm based on the coagulase and protein a gene polymorphisms
objective(s): staphylococcus aureus is an important bacterial pathogen responsible for a variety numbers of nosocomial and community acquired infections. biofilm formation is regarded as an important factor in the establishment of s. aureus infection. the contribution of the genetic background of s. aureus to biofilm formation is poorly understood. the aim of the present work was to genotype s....
متن کاملMolecular Typing of Acinetobacter Baumannii Clinical Strains in Tehran by Pulsed-Field Gel Electrophoresis
Background & Objective : Currently, Acinetobacter baumannii is an important nosocomial pathogen insofar as its hospital outbreaks have been described from various geographical areas. Since the discrimination of strains within a species is important for delineating nosocomial outbreaks, this study was conducted with the aim of genotyping the A. baumannii clinical strains in Tehran via the pulsed...
متن کاملComparison of pulsed-field gel electrophoresis and coagulase gene restriction profile analysis techniques in the molecular typing of Staphylococcus aureus.
Pulsed-field gel electrophoresis (PFGE) and coagulase gene restriction profile (CRP) analysis techniques were used to analyze 71 Staphylococcus aureus isolates recovered from nine food-borne disease outbreaks. Twenty-two PFGE profiles and 11 CRPs were identified, with discrimination indices of 0.86 and 0.72, respectively. In addition, the variable regions of the coagulase genes of 39 isolates w...
متن کاملTyping of Enterococcus faecium by polymerase chain reaction and pulsed field gel electrophoresis.
Polymerase chain reaction (PCR) with JB1 or REP consensus oligonucleotides and pulsed field gel electrophoresis (PFGE) were used to study genomic DNA extracted from 31 strains of enterococci. Eleven ATCC strains, representative of 11 species of Enterococcus, were initially tested by JB1-PCR, revealing that Enterococcus malodoratus and Enterococcus hirae presented identical banding patterns. Eig...
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ژورنال
عنوان ژورنال: Journal of Veterinary Diagnostic Investigation
سال: 2009
ISSN: 1040-6387,1943-4936
DOI: 10.1177/104063870902100614